Further to the methods for extraction, purification and analysis already published during PEPCY, a protocol format was established to allow methods to e easily accessible and improve documentation. These PEPCY Practical Guidelines (PPGs) aim to ensure standardized and traceable methods for use throughout-, and for life after-, PEPCY.
| No. | PPG Title |
Author |
|
1 |
Cytotoxicity test with intestinal and/ or hepatic cell lines. |
SJ Hoeger, DR Dietrich |
|
| 2 | Extraction and crude fractionation of extracts from lyophilized cyanobacterial cells for bioassays | S. Hoeger, M. Welker, J. Fastner, L.F. Morrison | PPG_UnivKon002 |
3 |
Analysis of anabaenopeptin B by high performance liquid chromatography with photodiode array detection. |
LF Morrison, GA Codd | |
4 |
Preparation of gravimetric cyanopeptide standards. |
LF Morrison, GA Codd | |
5 |
Extraction and purification of anabaenopeptin B. |
LF Morrison, S McInroy, GA Codd | |
6 |
Routine small-scale extraction of anabaenopeptins from lyophilised cyanobacterial biomass. |
LF Morrison, GA Codd, J Fastner | |
7 |
Routine small-scale extraction of cyanostatins (microginins) from lyophilised cyanobacterial biomass. | LF Morrison, GA Codd | |
8 |
Routine extraction of microcystin-LR, cyanopeptolin A, cyanopeptolin C and cyanopeptolin 970 from lyophilized cyanobacterial biomass | L Tonk, LF Morrison | |
9 |
Optimising extraction and HPLC analysis of peptides for cyanobacterial strains | M Welker | |
10 |
A manual for the use of in situ genetic techniques to quantify genotypes of cyanobacteria in freshwater under non-bloom conditions and to predict cyanopeptide occurrence under bloom conditions | Rainer Kurmayer, Eva Schober |