PEPCY-Toxic and Bioactive PEPtides in CYanobacteria

PEPCY Practical Guidelines (PPG)

Further to the methods for extraction, purification and analysis already published during PEPCY, a protocol format was established to allow methods to e easily accessible and improve documentation. These PEPCY Practical Guidelines (PPGs) aim to ensure standardized and traceable methods for use throughout-, and for life after-, PEPCY.

No.

PPG Title

Author

PDF

1

Cytotoxicity test with intestinal and/ or hepatic cell lines.

SJ Hoeger, DR Dietrich

PPG_UnivKon001

2 Extraction and crude fractionation of extracts from lyophilized cyanobacterial cells for bioassays S. Hoeger, M. Welker, J. Fastner, L.F. Morrison PPG_UnivKon002

3

Analysis of anabaenopeptin B by high performance liquid chromatography with photodiode array detection.

LF Morrison, GA Codd

PPG_UnivDun001

4

Preparation of gravimetric cyanopeptide standards.

LF Morrison, GA Codd

PPG_UnivDun002

5

Extraction and purification of anabaenopeptin B.

LF Morrison, S McInroy, GA Codd

PPG_UnivDun003

6

Routine small-scale extraction of anabaenopeptins from lyophilised cyanobacterial biomass.

LF Morrison, GA Codd, J Fastner

PPG_UnivDun004

7

Routine small-scale extraction of cyanostatins (microginins) from lyophilised cyanobacterial biomass. LF Morrison, GA Codd

PPG_UnivDun005

8

Routine extraction of microcystin-LR, cyanopeptolin A, cyanopeptolin C and cyanopeptolin 970 from lyophilized cyanobacterial biomass L Tonk, LF Morrison

PPG_UvA001

9

Optimising extraction and HPLC analysis of peptides for cyanobacterial strains M Welker

PPG_TUB1

10

A manual for the use of in situ genetic techniques to quantify genotypes of cyanobacteria in freshwater under non-bloom conditions and to predict cyanopeptide occurrence under bloom conditions Rainer Kurmayer, Eva Schober

PPG_AAS001